Detection of a specific pattern of hyaluronan oligosaccharides and their binding proteins in human ovarian tumour.
Identifieur interne : 001306 ( Main/Exploration ); précédent : 001305; suivant : 001307Detection of a specific pattern of hyaluronan oligosaccharides and their binding proteins in human ovarian tumour.
Auteurs : Srinivasa Prasad Kolapalli [Inde] ; Venatrao Nunna [Inde] ; Anil Thomas [Inde] ; Karuna Kumar Mortha [Inde] ; Shib Das Banerjee [États-Unis] ; Rajeev K. Boregowda [États-Unis]Source :
- Cell biochemistry and function [ 1099-0844 ] ; 2016.
Descripteurs français
- KwdFr :
- Acide hyaluronique (métabolisme), Femelle, Humains, Immunohistochimie, Masse moléculaire, Membrane cellulaire (métabolisme), Normes de référence, Noyau de la cellule (métabolisme), Oligosaccharides (métabolisme), Protéines de transport (métabolisme), Protéines tumorales (métabolisme), Tumeurs de l'ovaire (métabolisme).
- MESH :
English descriptors
- KwdEn :
- Carrier Proteins (metabolism), Cell Membrane (metabolism), Cell Nucleus (metabolism), Female, Humans, Hyaluronan Receptors (metabolism), Hyaluronic Acid (metabolism), Immunohistochemistry, Molecular Weight, Neoplasm Proteins (metabolism), Oligosaccharides (metabolism), Ovarian Neoplasms (metabolism), Reference Standards.
- MESH :
- chemical , metabolism : Carrier Proteins, Hyaluronan Receptors, Hyaluronic Acid, Neoplasm Proteins, Oligosaccharides.
- metabolism : Cell Membrane, Cell Nucleus, Ovarian Neoplasms.
- Female, Humans, Immunohistochemistry, Molecular Weight, Reference Standards.
Abstract
Tumour cells generate hyaluronan (HA) oligomers (O-HA) by an autocrine mechanism to regulate their own behaviour through receptor interaction, necessitating analysis of HA sizes and its receptor expression in tumour progression. In this study for the first time, we identified specific size of HA in malignant ovarian tumour compared to benign tumour tissue. Therefore, we prepared the identified HA probes and conducted multiplex and monoplex ligand blot analysis and Immunohistochemistry to identify their receptor expression and distribution. Although, HA recognized CD44 as principle receptors despite of size, multiplex analysis showed multiple receptor expression with distribution at the tumour cell surface. Furthermore, the HA 6-mer (major O-HA of ovarian tumour) pull down of tumour tissue proteins showed 120 kDa protein along with CD44 with over expression in the malignant tumour. Upon depletion of CD44 protein HA 6-mer showed a major 120 kDa protein with distribution at nuclear membrane, suggesting that this protein may play an important role in ovarian tumour progression. In summary, ovarian tumour cells of different grade showed heterogeneity in generation of HA oligomers and their interaction with specific receptors. Therefore, simultaneous analysis of O-HA and their receptors expression could serve as a prognostic indicator during tumorigenesis. Copyright © 2016 John Wiley & Sons, Ltd.
DOI: 10.1002/cbf.3179
PubMed: 27001487
Affiliations:
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In this study for the first time, we identified specific size of HA in malignant ovarian tumour compared to benign tumour tissue. Therefore, we prepared the identified HA probes and conducted multiplex and monoplex ligand blot analysis and Immunohistochemistry to identify their receptor expression and distribution. Although, HA recognized CD44 as principle receptors despite of size, multiplex analysis showed multiple receptor expression with distribution at the tumour cell surface. Furthermore, the HA 6-mer (major O-HA of ovarian tumour) pull down of tumour tissue proteins showed 120 kDa protein along with CD44 with over expression in the malignant tumour. Upon depletion of CD44 protein HA 6-mer showed a major 120 kDa protein with distribution at nuclear membrane, suggesting that this protein may play an important role in ovarian tumour progression. In summary, ovarian tumour cells of different grade showed heterogeneity in generation of HA oligomers and their interaction with specific receptors. Therefore, simultaneous analysis of O-HA and their receptors expression could serve as a prognostic indicator during tumorigenesis. Copyright © 2016 John Wiley & Sons, Ltd.</div></front></TEI><affiliations><list><country><li>Inde</li><li>États-Unis</li></country><region><li>Massachusetts</li><li>New Jersey</li></region></list><tree><country name="Inde"><noRegion><name sortKey="Kolapalli, Srinivasa Prasad" sort="Kolapalli, Srinivasa Prasad" uniqKey="Kolapalli S" first="Srinivasa Prasad" last="Kolapalli">Srinivasa Prasad Kolapalli</name></noRegion><name sortKey="Mortha, Karuna Kumar" sort="Mortha, Karuna Kumar" uniqKey="Mortha K" first="Karuna Kumar" last="Mortha">Karuna Kumar Mortha</name><name sortKey="Nunna, Venatrao" sort="Nunna, Venatrao" uniqKey="Nunna V" first="Venatrao" last="Nunna">Venatrao Nunna</name><name sortKey="Thomas, Anil" sort="Thomas, Anil" uniqKey="Thomas A" first="Anil" last="Thomas">Anil Thomas</name></country><country name="États-Unis"><region name="Massachusetts"><name sortKey="Banerjee, Shib Das" sort="Banerjee, Shib Das" uniqKey="Banerjee S" first="Shib Das" last="Banerjee">Shib Das Banerjee</name></region><name sortKey="Boregowda, Rajeev K" sort="Boregowda, Rajeev K" uniqKey="Boregowda R" first="Rajeev K" last="Boregowda">Rajeev K. 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